http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103983706-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_54b62fe8722e15b47c24080ac4e65287 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2014-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f92342f55ec303285033c9af1c94d4aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c608cd7cce86cd3319e0465d0da86e3d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b13c5617fe32e1a57788eb3365c816f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28349d95f19f69adf942eef4c9a19387 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b486fdf2fdabbced20d9d174d2bc1b0d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_758b211c28777592afd606276491ff62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c29eba35c61d73421131ded64dcaa406 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad9e098e1d7e92da7f1530600d414e39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4931ea21857ff42bbac8cbdc3d0eb528 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c87c47540164cc207def8fe07b62d23 |
| publicationDate | 2015-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103983706-B |
| titleOfInvention | Precolumn derivatization and high-efficiency liquid-phase fluorescence detection method for tetrodotoxin and kit based on the method |
| abstract | The invention discloses a precolumn derivatization and high-efficiency liquid-phase fluorescence detection method for tetrodotoxin. The method includes following steps: (1) preparing a sample solution of a sample to be measured and a standard substance solution of tetrodotoaxin; (2) adding 0.2 ml of a pH maintenance solution to 0.4 ml of the sample solution for controlling pH of the sample solution at a range between 11.6 and 11.9, then adding 0.1 ml of a derivatization agent 1 and 0.1 ml of a derivatization agent 2, mixing uniformly, carrying out a water-bath heating process for 20 min and then carrying out a cooling process to room temperature, adding 0.1 ml of a stopping solution for enabling the pH of the sample solution to be neutral, and then carrying out a then diluting to volume with ultrapure water, adding the standard substance solution of the tetrodotoxin to the sample solution, mixing uniformly, and then carrying out a fluorescence derivatization process to the sample solution; and (3) with a liquid-phase chromatograph instrument equipped with a fluorescence detector, analyzing the sample solution which is subjected to the fluorescence derivatization process in the step (2) and the standard substance solution of the tetrodotoxin to detect the tetrodotoxin qualitatively and quantitatively in the sample solution. The invention also discloses a kit which is suitable for the method. The method is used for detecting the tetrodotoxin in aquatic products qualitatively and quantitatively and is reliable in result and low in detection limit. |
| priorityDate | 2014-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.