http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103983621-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
filingDate | 2014-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103983621-B |
titleOfInvention | The fluorescence detection method of flavone component in a kind of hawthorn |
abstract | The invention provides the fluorescence detection method of flavone component in a kind of hawthorn: first extract hawthorn with methyl alcohol and obtain hawthorn extract, as sample liquid after dilution, carry out Capillary Electrophoresis fluoroscopic examination, fill micro emulsion buffer solution in capillary column, the composition of described micro emulsion buffer solution is: the buffer salt of zwitterionic surfactant 3 ~ 12mg/mL, cosurfactant 55 ~ 70mg/mL, ethyl acetate 4.5 ~ 6mg/mL, 2.5 ~ 10mmol/L, pH value are 8 ~ 10.Then the content of each flavone component in hawthorn is calculated according to the typical curve of reference substance.The present invention is directed to the deficiency of existing chromatographic technique, provide a kind of prepare simple, dissolving power is good, viscosity is low, the micro emulsion damping fluid of good stability, and in damping fluid the neutral zwitterionic surfactant of creative use; Meanwhile, the present invention preferentially uses highly sensitive, reproducible detector of fluorescence induced by light-emitting diode, and achieves significant effect in flavones context of detection. |
priorityDate | 2014-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.