http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103981144-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 |
filingDate | 2014-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103981144-B |
titleOfInvention | The preparation method of autoserum antigen sensibilization DC CIK cells |
abstract | The invention discloses a kind of preparation method of autoserum antigen sensibilization DC CIK cells, the preparation method of the autoserum antigen sensibilization DC CIK cells, including step:A)The preparation of autoserum antigen;B)The preparation of PMNC;C)The separation and culture of DC;D)The induced amplification of CIK cell;E)DC is co-cultured with CIK cell and is prepared DC CIK cells.The DC CIK of the method for the invention culture its proliferation activity to CIK and kill tumor activity and substantially increase compared with DC CIK prepared by cellar culture, and its proliferation times has averagely reached 200 250 times, is 23 times of classical culture protocols;Tumor activity is killed during experiment in vitro and reaches 70 80%, hence it is evident that higher than the DC CIK cells of conventional method culture.This method operation is simple, and condition is easily-controllable, and the requirement to equipment is relatively low, and the DC CIK cells propagation for being obtained is in hgher efficiency. |
priorityDate | 2014-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.