http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103969321-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
filingDate | 2013-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103969321-B |
titleOfInvention | Based on the Identification of Fusion Protein of the efficient enzymatic isolation method of immobilised enzymes original position and the method for mass spectrum imaging |
abstract | The invention belongs to biochemical analysis field, relate to a kind of immobilised enzymes in-situ enzymolysis histotomy and realize Identification of Fusion Protein and be applied to the new method of mass spectrum imaging.The graphene oxide that the present invention utilizes surface to have the hydrophilic radicals such as hydroxycarboxyl group fixes trypsase, by improving enzymolysis efficiency, realize the efficient enzymolysis of the original position of protein and qualification on histotomy, and be applied to mass spectrum imaging technology, determine the distribution of protein in section.Carrier material graphene oxide selected by the present invention has extremely strong adsorptive power, can adsorb trypsase at short notice, utilizes confinement effect to greatly improve tryptic enzymolysis efficiency, effectively facilitates the qualification of histotomy in-situ enzymolysis and albumen.The present invention has that step is simple, easy to operate, the feature such as rapidly and efficiently, can be widely used in proteomics association area. |
priorityDate | 2013-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 195.