http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103966357-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2304-10 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-06 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06 |
filingDate | 2013-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103966357-B |
titleOfInvention | A rapid quantitative detection method for viruses in coelomic fluid of sea cucumbers |
abstract | The invention discloses a rapid quantitative detection method for viruses in the body cavity fluid of japonicus japonicus, comprising the following steps: 1) extraction of the body cavity fluid of japonicus japonicus; 2) ultrasonic crushing of body cavity fluid cells, freezing and thawing, and release of virus particles; 3) body cavity Liquid filtration, made of aluminum oxide filter membrane containing virus; 4) dark staining, film production, observation, counting. The invention can quickly and directly detect the virus in the imitation sea cucumber without killing the imitation sea cucumber, and the detection process can be completed within 5 hours. It has the characteristics of simple, fast and accurate operation, and can be used for imitation sea cucumber seed Rapid screening of breeding species. |
priorityDate | 2013-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Predicate | Subject |
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isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID452902837 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID11857532 |
Total number of triples: 17.