http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103966294-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-78 |
| filingDate | 2013-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103966294-B |
| titleOfInvention | Biological active collagen extracting method |
| abstract | The present invention relates to a kind of biological active collagen method of purification, including：Pre-treatment step, is made fragment standby to fresh ox heel string pre-treatment；Acid system slightly puies forward step, takes 1 weight portion fragment and adds 4 ~ 8 parts by weight acetic acid or citric acid solution and stir, and it is standby then to take supernatant after coarse filtration, high speed centrifugation coarse filtration liquid；Enzyme process goes end group step, adjusts supernatant pH to 9.0, and the ficin for adding 0.1 ~ 0.3% weight portion carries out enzymolysis, digestion；Gradient salting-out step, carries out many gradients to the solution after enzymolysis with precipitating reagent and saltouts treatment, and acquisition is dissolved with the solution of collagen；Dialysis step, the solution that will be dissolved with collagen is fitted into bag filter, is successively that extracellular fluid dialysis are dialysed with acetum and pure water, obtains the collagen stoste with bioactivity.The present invention combines acid system and the existing collagen extraction method of two kinds of enzyme process, had not only overcome acid system low yield but also had overcome the halfway shortcoming of enzymatic hydrolysis, and uses non-animal source protease, reduces on animal derived risk. |
| priorityDate | 2013-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.