http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103966233-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 |
filingDate | 2014-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103966233-B |
titleOfInvention | A kind of sugarcane callus colour developing controlling gene ScSN and application thereof |
abstract | The invention provides a kind of sugarcane callus colour developing controlling gene ScSN and application thereof, according to corn anthocyanidin transcription factor gene <i>SN</iGreatT.G reaT.GT(GenBank:X60706.1) upstream, downstream sequence design primer, utilize Homology-based cloning, obtain the full length sequence of anthocyanidin transcription factor gene <i>ScSN</iGreatT .GreaT.GT from sugarcane.Does is sugarcane <i>ScSN</iGreatT .GreaT.GT gene open reading frame length 1722? bp, 573 amino acid of encoding.The coding region sequence of sugarcane <i>ScSN</iGreatT .GreaT.GT gene is carried out BLAST analysis in NCBI, with the BLAST Homology search of numerous corn anthocyanidin genes involved, E value equals zero, and describing cloned gene is sugarcane anthocyanidin synthesis related gene really.<i>Kpn</iGreatT. GreaT.GT I is added respectively at <i>ScSN</iGreatT .GreaT.GT sequence two ends, <i>Sal</iGreatT. GreaT.GT I restriction enzyme site, build plant expression vector pCAMBIA1301-35SN-ScSN, again by biolistic bombardment Transformation of Sugarcane callus, the callus color transition transformed is garnet, the gene that further confirmation is cloned has the function that sugarcane callus is developed the color. |
priorityDate | 2014-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.