http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103966220-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
filingDate | 2014-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103966220-B |
titleOfInvention | The root-specific promoter of synthetic and application thereof |
abstract | The present invention relates to " root-specific promoter of synthetic and application thereof ", belong to biological technical field.The root-specific promoter of synthetic, named SRS1, its nucleotide sequence is as shown in SEQ ID NO1, overlapping PCR method is utilized to add 35S promoter subregion and Omega sequence, the sequence of synthesis is SRSO1, carrier (containing GUSplus reporter gene) based on pCAMB2300.1, the CaMV35S promoter on pCAMBIA2300.1 is replaced respectively by the promoter of synthetic, obtain expression vector p2300.1 SRSO1, after converting Agrobacterium, proceed to Nicotiana tabacum L. is verified, show that promoter SRS1 has root-specific expression characterization.Promoter SRS1 of this synthetic, enriches the kind of root specific expression promoter, and the root that can be used for Insect-Resistance Gene of Plant is expressed, and prevents and treats subterranean pest-insect. |
priorityDate | 2014-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.