http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103954701-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2014-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103954701-B |
| titleOfInvention | HPLC is utilized to detect the method for β-Isosorbide-5-Nitrae-xylose transferase activity in xylan synthesis |
| abstract | The present invention discloses a kind of method utilizing HPLC to detect β-Isosorbide-5-Nitrae-xylose transferase activity in xylan synthesis.The present invention, by extracting plant microcapsule, utilizes the β-Isosorbide-5-Nitrae-xylose transferase existed in microcapsule, carries out Xyln n -AA and the addition reaction of UDP-Xyl, then utilize HPLC to carry out the detection of addition compound product, inquire into the activity of XylT in plant.Due to the key enzyme that XylT is xylan synthesis, xylan is the cellulosic principal ingredient of biomass half, and the synthesis mechanism understanding xylan is conducive to utilizing biomass half cellulose better.The present invention is for further investigation xylan synthesis mechanism and regulate xylan composition in hemicellulose to provide a kind of approach reliably, can be as required, by screening xylan synthesis deficiency plant, the gene that research regulates xylan to be formed, or by regulating the key gene affecting XylT synthesis, meet the demand to hemicellulose different content in material. |
| priorityDate | 2014-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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