http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103952495-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
filingDate | 2014-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103952495-B |
titleOfInvention | A kind of LAMP detection method of mandarin fish infectious spleen and kidney necrosis virus |
abstract | The invention discloses a kind of LAMP detection method of mandarin fish infectious spleen and kidney necrosis virus, the method is 6 zone design 4 species-specific primers for infectious spleen and kidney necrosis virus specific fragment, utilize LAMP rapid amplifying masterplate DNA specific regions, by adding dyestuff, by visual color change just energy judged result under natural light, whole reaction is no more than 2h, again because LAMP is waterfall type amplification, therefore its amplification efficiency is very high, sensitivity is 100 times of regular-PCR, and without the need to the detecting instrument of complexity and testing process, simple to operate, on-the-spot detection in real time can be realized, therefore the present invention is that a kind of detection is quick, highly sensitive, easy and simple to handle, cost is low, detected result is easy to the LAMP detection method of the infectious spleen and kidney necrosis virus judged. |
priorityDate | 2014-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.