http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103952492-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3b597bb5b93feed1656da75a114b7a05 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
filingDate | 2014-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e83e30a7e275d82b7be7de02f78d2bc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_257ae174b54d61c84acfb10e4fc7a0c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_572144dc01e5b312b5a9752a5094435f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c45c8b33aedd6f5f4291e3d3ad8f16f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa07736231217e6c2e4aaea765ff47db |
publicationDate | 2014-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103952492-A |
titleOfInvention | Real-time fluorescent PCR detection method of Didymella ligulicola |
abstract | The invention discloses a real-time fluorescent PCR detection method of Didymella ligulicola. The method comprises the following step: by taking DNA of a to-be-tested sample as a template, carrying out PCR amplification by means of a forward primer DL1, a reverse primer DL2 and a fluorescent probe Probe-DL; if specific amplification happens, showing that the to-be-tested sample carries the Didymella ligulicola, wherein the forward primer DL1 is 5'-CAACACTTAAACCCTTTGTAATTGA-3, the reverse primer DL2 is 5'-GGACGTCGTCGTTTTGTTGT-3, and the fluorescent probe Probe-DL is 5'-FAM-ACAACTTTCAACAACGGATC-TAMRA-3. The method for detecting Didymella ligulicola has the characteristics of speediness, sensitivity, high specificity and the like. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109628550-A |
priorityDate | 2014-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.