http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103952492-A

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filingDate 2014-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e83e30a7e275d82b7be7de02f78d2bc
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publicationDate 2014-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103952492-A
titleOfInvention Real-time fluorescent PCR detection method of Didymella ligulicola
abstract The invention discloses a real-time fluorescent PCR detection method of Didymella ligulicola. The method comprises the following step: by taking DNA of a to-be-tested sample as a template, carrying out PCR amplification by means of a forward primer DL1, a reverse primer DL2 and a fluorescent probe Probe-DL; if specific amplification happens, showing that the to-be-tested sample carries the Didymella ligulicola, wherein the forward primer DL1 is 5'-CAACACTTAAACCCTTTGTAATTGA-3, the reverse primer DL2 is 5'-GGACGTCGTCGTTTTGTTGT-3, and the fluorescent probe Probe-DL is 5'-FAM-ACAACTTTCAACAACGGATC-TAMRA-3. The method for detecting Didymella ligulicola has the characteristics of speediness, sensitivity, high specificity and the like.
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