http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103952441-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate | 2014-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103952441-B |
| titleOfInvention | The construction method of 1.3 copy C genotype HBV transgenic mices |
| abstract | The invention provides the construction method of a kind of 1.3 copy C genotype HBV transgenic mices.Described method is to prepare site-directed integration 1.3 by blastaea injection ES cell to copy C genotype hepatitis B virus (Hepatitis B virus, HBV) transgenic mice.First build site-directed integration 1.3 and copy the expression vector of c-type HBV, then use electricity transduction method for transformation in recombinant plasmid transformed to ES cell, the ES cell of site-directed integration restructuring is obtained after screening and cultivation, again the ES cell of site-directed integration restructuring is expelled in blastaea after being digested to individual cells, then blastaea is transplanted in pseudo-fetus dams uterus.The method at home and abroad takes the lead in establishing the transgenic mice of 1.3 copy c-types HBV, is applied to targetedly in hepatitis B Related Research Domain, for China's hepatitis B prevention and Therapy study more preferably animal model. |
| priorityDate | 2014-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.