| abstract |
The invention discloses the preparation method of a kind of sericin hydrogel, first the method weighs bombyx mori silk fibroin deletion form mutating variety Bombyx bombycis, extracts through LiBr or LiCl, and dialysis purification obtains the sericin aqueous solution that mass percentage concentration is 0.1～4% of non-degradable；Then sericin aqueous solution is concentrated into 1.5～10.0%, sericin aqueous solution after concentrating adds cross-linking agent (cross-linking agent of every 1mL sericin aqueous solution addition 2～500 μ L), fully mixing is placed at 4～45 DEG C 5 seconds～36 hours, obtains hydrogel.This hydrogel has biological activity and multifunctionality, can be as carrying somatomedin, medicine and cell carrier, can be applicable to the reparation of multiple soft tissue injury and the treatment of disease, include but not limited to skin injury, muscle injury, blood vessel injury, nerve injury, myocardial damage etc.. |