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filingDate 2014-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2016-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2016-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103951630-B
titleOfInvention The tree-shaped haptens of a kind of direct-detection Furaltadone metabolite AMOZ, tree-shaped antigen and application thereof
abstract The present invention discloses the tree-shaped haptens of a kind of direct-detection Furaltadone metabolite AMOZ, tree-shaped antigen and application thereof. Tree-shaped haptens is that the active amino end that the glyoxalic acid derivative of AMOZ is coupled at four branch's polyamide-amides obtains, and prepares artificial antigen based on this tree-shaped haptens, then prepares antibody with artificial antigen. The direct high quality antibody for AMOZ can be obtained by the artificial antigen of the present invention, antibody tire as 1:64000, does is the lowest detection of AMOZ limited to 0.112 by the chemiluminescence enzyme linked immune analytic method (CLEIA) set up based on this antibody? ng/mL, does is 503nhibiting concentration 15.26? ng/mL, the detection of AMOZ can be directly used in, overcome the AMOZ haptens of traditional scheme, that artificial antigen prepares the antibodies specific is poor, can not the shortcoming of Direct Recognition AMOZ itself. Being with a wide range of applications in the residue detection of the tree-shaped haptens of the present invention, artificial antigen and antibody AMOZ in food, the preparation thinking of this haptens can be offered reference for the hapten design of other similar micromolecular compounds simultaneously.
priorityDate 2014-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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