http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103941004-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5ed85c5a886b8df4923d9a2596262902 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56911 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 |
| filingDate | 2014-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a0fa2d7fa28c66170fb652e443bb65f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0a98a8f17b15387f9c2ebf207e30d45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccadf2611d9abf11004bb5b304aac862 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cca9c6ac949d4d4fa17d20b22848ff45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e5118e315b5d858c685af62f015de60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_547a10d9b9f32085be2b30d87ca5243d |
| publicationDate | 2015-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103941004-B |
| titleOfInvention | Clostridium perfringens alpha toxin double-antibody sandwich ELIS quantitative determination method |
| abstract | The invention relates to a clostridium perfringens alpha toxin double-antibody sandwich ELIS quantitative determination method. According to the clostridium perfringens alpha toxin double-antibody sandwich ELIS quantitative determination method, alpha toxin protein obtained via prokaryotic expression is taken as an immunogen; monoclonal antibodies obtained via hybridoma technique are taken as detection antibodies and capture antibodies; reaction conditions are optimized via experiments; alpha toxin protein samples with a series of concentration are used for construction of a standard curve; the double-antibody sandwich ELIS method is established; and indexes of the double-antibody sandwich ELIS are verified. The clostridium perfringens alpha toxin double-antibody sandwich ELIS quantitative determination method specifically comprises following steps: (1) prokaryotic expression of alpha toxin; (2) preparation of anti-alpha toxin monoclonal antibodies; (3) establishment of the double-antibody sandwich ELIS method; (4) establishment of the standard curve; and (5) performance evaluation on the double-antibody sandwich ELIS method. The clostridium perfringens alpha toxin double-antibody sandwich ELIS quantitative determination method is excellent in specificity, and high in sensitivity and stability, is fast and convenient, and can be used for effective quantitative determination of alpha toxin in A-E type clostridium perfringens cultural supernatants; and the high-efficient detection method is provided for alpha toxin determination. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106093404-A |
| priorityDate | 2014-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.