http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103937821-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 |
| filingDate | 2014-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103937821-B |
| titleOfInvention | Nitrilase gene and prokaryotic expression and immobilization technology thereof |
| abstract | The invention relates to a nitrilase gene and a prokaryotic expression and immobilization technology thereof, belonging to the technical field of prokaryotic expression and immobilization of chemical enzymes. The prokaryotic expression and immobilization technology comprises the steps of cloning nitrilase to a nitrilase gene by taking a clostridium thermoceuum genome as a template by utilizing a PCR (Polymerase Chain Reaction) technology, carrying out prokaryotic expression on the nitrilase through an Escherichia coli expression system to obtain a nitrilase gene contained recombinant plasmid pET-28a-nit, converting the recombinant plasmid pET-28a-nit into Escherichia coli BL21 to obtain a recombinant strain BL21 (pET-28a-nit), inducing the expression of the recombinant strain to obtain recombinant nitrilase; connecting the nitrilase gene and a high-copy shuttle plasmid connected with a spore capsid protein gene cotG to construct a recombinant plasmid Phs-cotG-nit for surface display, transferring the plasmid into a bacillus subtilis strain DB403 to obtain a recombinant strain DB403 (pHS-cotG-nit), and inducing the surface of a recombinant spore generated by the recombinant strain to display the nitrilase. According to the invention, the nitrilase is firstly immobilized by utilizing a spore surface display technology; in addition, the method is not only capable of ensuring higher structural stability of the nitrilase, but also beneficial to the recycle of the nitrilase after the nitrilase is catalyzed, and the spore with the nitrilase displayed on the surface can be recycled through centrifuging. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107254429-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107254429-B |
| priorityDate | 2014-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 680.