http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103937778-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-14 |
| filingDate | 2014-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103937778-B |
| titleOfInvention | A kind of preparation method of immobilized-lipase |
| abstract | A kind of method that the invention provides immobilized-lipase, with the magnetic Fe of aminofunctional 3 O 4 Lipase is fixed on its surface as carrier by covalent bond by@C nano particle.It is specifically divided into two kinds of methods: one is by the magnetic Fe of glutaraldehyde one terminal aldehyde group Yu aminofunctional first with necleophilic reaction 3 O 4 After the amino of@C nano particle surface combines, then magnetic Fe will be fixed on 3 O 4 The glutaraldehyde other end aldehyde radical of@C nano particle surface combines with the amino of lipase；Two is to utilize succinic anhydride by magnetic Fe 3 O 4 The amino of@C nano particle surface is converted into carboxyl, then after the amount of material is than the N for 1:0.5 ~ 2, N dicyclohexylcarbodiimide and N hydroxysuccinimide mixture activation, utilizes amidation process at Fe 3 O 4 @C nano particle surface fixed fat enzyme.The preparation method of the present invention is simple, gentle, and lipase supported quantity is many, and immobilized-lipase enzyme is lived and enzyme is lived response rate height, good stability, can circulate easily and re-use, and effectively reduces real cost of production, is with a wide range of applications. |
| priorityDate | 2014-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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