http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103937766-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-685 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 |
filingDate | 2014-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103937766-B |
titleOfInvention | Recombination gene and method for increasing aspergillus niger expressed saccharifying enzyme |
abstract | The invention belongs to the field of gene engineering, and discloses a recombination gene and a method for increasing aspergillus niger expressed saccharifying enzyme. The method for increasing aspergillus niger expressed saccharifying enzyme disclosed by the invention is as follows: by means of a homologous recombination method, a saccharifying enzyme expression cassette and selectable marker genes are integrated in an aspergillus niger An12g08830 gene locus in a targeted manner; then, saccharifying enzyme is obtained by recombining aspergillus niger through conventional resistance screening and fermentation cultivating. According to the invention, after the saccharifying enzyme expression cassette is integrated in the aspergillus niger An12g08830 gene locus in a targeted manner through the improved homologous recombination method, the conversion efficiency and the saccharifying enzyme expressing activity are obviously increased; furthermore, a lot of processes for screening converters are omitted. |
priorityDate | 2014-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 882.