http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103937742-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2014-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103937742-B |
| titleOfInvention | One activates CD4 simultaneously+& CD8+the cultural method of the immunocyte of T cell |
| abstract | The invention belongs to tumor biotherapy field, relate to one and activate CD4 simultaneously + & CD8 + The immunocyte cultural method of T cell.The mononuclearcell of separator well is placed in containing rhIL 12, IL 4 part, IL 10 part culture fluid in, add levamisole after cultivating 8 12 hours to continue to cultivate 8 12 hours, add Anti CD3McAb and rhIL 2, continue to cultivate 23 days, then being alternately added the culture medium containing rhIL 2 and containing Anti CD3McAb, the culture medium of rhIL 2, middle interval incubation time is 23 days.Preparation cytokine compound scheme be: from cultivation start after the 3rd 14 day collect cell supernatant, by low temperature ultracentrifugation, ultra-filtration centrifuge tube ultrafiltration, microporous filter membrane microfiltration, filtrate is natural cytokines mixture.The cultural method of the present invention, cell can keep continuous proliferation, up to 30 days.NCM comprises cytokine profiles, and human immunocyte can be stimulated to play autoimmune anti-tumor activity. |
| priorityDate | 2014-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 223.