http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103926248-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N31-22 |
filingDate | 2014-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103926248-B |
titleOfInvention | Homocysteine (Hcy) assay method and mensuration kit |
abstract | The invention provides a kind of assay method of homotype semicystinol concentration investigating, principle is that oxidized form Hcy is converted to free Hcy, and free Hcy generates L-cystathionine with serine reaction under CBS catalysis.L-cystathionine generates again Hcy, pyruvic acid and NH3 under CBL catalysis.The pyruvic acid that this circular response generates can detect with lactate dehydrogenase L DH and NADH, and the speed that NADH is transformed into NAD is directly proportional to Hcy content in sample.NADH is measured at 340nm placen + changing down can record the content of homocysteine.The present invention also provides a kind of mensuration kit of high sensitivity homotype semicystinol concentration investigating. |
priorityDate | 2014-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 357.