| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_01c0ec9a3d5dd4264d8292aa54ee9a5a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2012-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a9d697326c16f662e51742958f3123b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69ac5dba26c7f33ec7c527ab7b9ee067
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94e84b0663f1e4826ceb29007d8c939c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff467ac16a39dbcda7f826bc784d97b7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2de0e704fa34190629be9d2c7c154384
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73ac20d775e30df01932a824145b893c |
| publicationDate |
2014-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-103911424-A |
| titleOfInvention |
Kit and PCR amplification method for detection of one pathogenic mutation site of male infertility Dmc1 gene |
| abstract |
The invention discloses a kit and a PCR amplification method for detection of one pathogenic mutation site of a male infertility Dmc1 gene. The method is characterized in that a modified mutation-detection primer resistant to 3'-5' exonuclease enzyme digestion is used in a PCR reaction in which a high-fidelity DNA polymerase participates, so that the specificity of PCR is improved; and the existing condition of a gel electrophoresis product band can be directly observed to judge the genotype of the Dmc1 gene corresponding mutation site. The detection kit has low requirements on instruments and equipment, is simple in operation, is economic, has no need of sequencing, and can be operated in common molecular biology laboratories. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105316396-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105296603-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107619862-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105316394-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107619862-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105316395-A |
| priorityDate |
2012-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |