http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103911417-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 |
| filingDate | 2014-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103911417-B |
| titleOfInvention | Fermentable prepares the method for Guangchang Nymphaea alba blood pressure lowering polypeptide |
| abstract | A kind of fermentable prepares the method for Guangchang Nymphaea alba blood pressure lowering polypeptide, is pulverized by white lotus, and the formula (kg/L) of fermentation liquid culture medium is: 3 6% white lotus powder, 1 5% carbon source, water, natural ph；Take cell age and be in the activated strains of 28 44h, Bacillus licheniformis ND is diluted in 1mL containing 1*10 7 After CFU, seed liquor being inoculated in fermentation liquid culture medium by 2 6% inoculum concentrations, 37 DEG C, 125r/min shaking table is cultivated, and cultivates 32 52h；Taking fermentation liquid, under 10000r/min, centrifugal 20min, is precipitated as thalline and unhydrolysed white lotus component, takes supernatant 0.45 μm filtering with microporous membrane, and filter vacuum is dried；Under polydextran gel Sephadex G25, it is carried out isolated and purified, according to the OD of each collecting pipe after dissolving with distilled water 220 , tunning, after gel chromatography, collects the 2nd absworption peak vacuum drying.Present invention process is simple, low cost, it is thus achieved that mixed polypeptide molecular weight majority less than 1500D, and its hypotensive activity is more preferable than the polypeptide that use papain obtains. |
| priorityDate | 2014-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.