http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103911388-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 |
| filingDate | 2013-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103911388-B |
| titleOfInvention | Recombinate the production technology of Exenatide |
| abstract | The present invention discloses the production technology of restructuring Exenatide, including the step of the step of the step of structure engineering bacteria, fermentation expression engineering bacteria and purifying destination protein；Wherein, present invention additionally comprises the step of carrying out amidatioon to Exendin 4 and process.Present invention utilizes pET 31b (+) itself can be extensive, the advantage of the production polypeptide of high yield, remain its powerful promoter, overcome the shortcoming that the protein product in current other production technologies easily forms inclusion body, the cutting characteristic of enterokinase make use of to be cut in the N-terminal of destination protein, the protein after cutting is entirely the Exendin 4 with native sequences.By engineering bacteria fermentation expression and destination protein obtain after purification recombinate Exenatide.Yield can reach more than 500mg/ liters, improve 20 times than current state-of-the-art process yields, and be soluble protein. |
| priorityDate | 2013-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 159.