http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103911339-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2013-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103911339-B |
| titleOfInvention | A kind of serum-free fibroblast culture medium and preparation method thereof |
| abstract | A kind of serum-free fibroblast culture medium and preparation method thereof, basic medium of the present invention is DMEM substratum or its mixing with F12 substratum; Additives comprises hormone, turns metalloprotein, glutamine, lipid enriching agent, antioxidant, purine, carbohydrate and cell growth factor.Compared with prior art advantage of the present invention is: do not add animal derived or recombinant albumin, avoids the potential safety hazard that may carry, and reduces culture medium cost; Be applicable to fibroblastic primary and Secondary Culture, and keep good cellular form, with state and the functional similarity of serologic group cell; Do not need to adopt anchoring factor bag quilt, primary 24h adherent rate > 60%, go down to posterity 24h adherent rate > 80%, with serologic group cell indifference; The needs that repeatedly go down to posterity can be met, and after reaching for 15 generations, upgrowth situation still keeps similar to serologic group cell state; Cultivated from containing directly proceeding to the present invention serum free culture system by inoblast, cell can smooth adherent stretching, extension, does not need adaptive process gradually. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107937335-A |
| priorityDate | 2013-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 265.