| abstract |
The present invention relates to genetically modified yeast cells, it comprises :-at least one recombinant promoter is operably connected at least one gene of coded polypeptide or protein, described polypeptide or protein support the biosynthesis of described intracellular polypeptides or protein, at least one gene described is positioned at the native genomic locus of the wild type yeast cells without genetic modification, wherein the naturally occurring promoter of at least one gene of the encoding human supportive polypeptide of synthesis or protein is inactivated by least one sudden change in described naturally occurring promoter, with,-secretion box, it comprises the recombinant nucleic acid molecules of coding proteins of interest matter or polypeptide, and the method using this kind of Hemapoiesis recombinant protein interested or polypeptide. |