http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103900883-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
| filingDate | 2014-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103900883-B |
| titleOfInvention | Cashmere goat skin is for the preparation method of the ultrathin section of transmission electron microscope observing |
| abstract | The present invention relates to the research of animal histology's technical field, be specifically related under transmission electron microscope observe making and the colouring method of ultrathin section used.A kind of Cashmere goat skin is for the preparation method of ultrathin section of transmission electron microscope observing, and it is mainly characterized by and includes step and be: (1) sampling；(2) sample is fixing: the skin sample rinsing that step (2) is fixed by (3)；(4) insert the osmic acid 24 DEG C of 2%, fix 3 5h；(5) phosphate buffer using 0.1 0.2mol/L rinses 3 times, each 10 15 min；(6) dehydration；(7) embedding: be impregnated with 3 6h for the first time in by the pure embedding medium of 1:1 and 100% acetone mixture, be then impregnated with 10 12h in pure embedding medium.It is an advantage of the invention that flow process is simple, easy to operate, it is thus achieved that section complete, picture structure is clear.It is applicable to the research to Cashmere goat skin morphosis of the laboratory researcher. |
| priorityDate | 2014-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.