http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103898223-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103898223-B |
| titleOfInvention | A kind of method using many reference genes combination research diversiform-leaved poplar gene |
| abstract | The invention discloses a kind of method using many reference genes combination research diversiform-leaved poplar gene, comprise the steps: that (1) extracts the total serum IgE of diversiform-leaved poplar, reverse transcription is cDNA, as the template of PCR; (2) use is more than or equal to 9 reference genes, does design Auele Specific Primer select SYBR on real-time fluorescence quantitative PCR instrument? Green method is reacted, and obtains CT value; (3) by the PV value computing function of formula and geNorm software, be the number of threshold value determination reference gene with 0.15; (4) select reference gene according to required number, carry out qRT-PCR reaction together with goal gene, by the CT value that obtains by geNorm software, calculate goal gene under each process, combine the delta data obtaining relative expression quantity with many reference genes.The method of the invention, compared with traditional single reference gene method, can obtain more reliable conclusion. |
| priorityDate | 2014-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 193.