http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103898083-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A62D2101-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A62D2101-26 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-05001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A62D3-02 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A62D101-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A62D101-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A62D101-26 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A62D3-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2014-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103898083-B |
| titleOfInvention | A kind of novel hydrolytic enzyme superfamily amidase Azl13 and preparation and application thereof |
| abstract | The invention discloses a kind of novel nitrilase superfamily amidase Azl13 and preparation and application thereof.The aminoacid sequence of this amidase Azl13 is such as shown in SEQ ID NO.1, and it has aryl acylamidase activity.The nucleotide sequence of the encoding gene of amidase Azl13 is such as shown in SEQ ID NO.2.Build recombinant expression plasmid by being connected to by the encoding gene of amidase Azl13 in prokaryotic expression carrier, then recombinant expression plasmid is transformed in escherichia coli, obtain amidase Azl13 through IPTG abduction delivering.The amidase Azl13 of the present invention can be used for administering aniline Amide class environmental contaminants, particularly Stam F-34.The present invention adopts genetic engineering bacterium to produce amidase Azl13, and with short production cycle, cost is relatively low, non-environmental-pollution, is suitable for large-scale culture；The amidase Azl13 of the present invention is with a wide range of applications in environment insecticide pollution improvement. |
| priorityDate | 2014-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 109.