http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103898060-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2012-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103898060-B |
| titleOfInvention | The screening method of HAT semisolid screening culture medium and hybridoma |
| abstract | The invention discloses a kind of HAT semisolid screening culture medium, HAT semisolid screening culture medium described in every 100mL is become to be grouped into by following: the dual anti-solution of 50 × HAT stock solution of 2mL, the foetal calf serum of 20mL, the 100 × penicillin of 1mL and Streptomycin sulphate, 100 × B-cell stimulating factor solution of 1mL, 50 × hybridoma cell fusion Cloning Factor solution of 2mL, the antigenic solution of 100 × isothiocyanic acid element mark of 1mL and the methylcellulose gum semisolid medium of surplus.The invention also discloses a kind of screening method using the hybridoma of HAT semisolid screening culture medium, fused cell is colony growth on semisolid medium, separated from one another, each clone is formed by individual cells, the hybridomas grew avoiding not secretory antibody affects the growth of the hybridoma of secretory antibody very soon, compared with traditional limiting dilution assay, screening efficiency is higher. |
| priorityDate | 2012-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 415.