http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103897018-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2012-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103897018-B |
| titleOfInvention | The extracting method of soya seeds gross protein and special agent thereof |
| abstract | The invention discloses extracting method and the special agent thereof of soya seeds gross protein.Described method comprises the steps: 1) take soya seeds and solution S 1 in the ratio mixing extraction protein of 1g:3ml, collection all substances acquisition mixture first；2) in the described mixture first of step 1), add the solution S 2 identical with solution S described in step 1) 1 volume and extract protein again, collect all substances and obtain mixture second；3) by step 2) described mixture second be centrifuged, take supernatant trichloroacetic acid precipitation, it is thus achieved that described soya seeds gross protein.Described solution S 1 and S2 is special agent.Using the inventive method and reagent to extract soybean seed protein quality preferable, purity is higher, can get 900 protein sites when carrying out Two-dimensional Electrophoresis Analysis, and some low-molecular-weights, low-abundance protein are high-visible.363 protein sites can be identified by mass spectrum, be significantly larger than it has been reported that additive method.The present invention has great using value to the research of plant proteomics research. |
| priorityDate | 2012-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.