http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103884576-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-40 |
| filingDate | 2014-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103884576-B |
| titleOfInvention | Method for extracting and separating whole protein of rat hippocampus, suitable for dimensional electrophoresis |
| abstract | The invention discloses a method which is used for extracting and separating whole protein of rat hippocampus and is suitable for dimensional electrophoresis. The method comprises the following steps: (1) placing a rat in frozen artificial cerebrospinal fluid; (2) pouring liquid nitrogen in a mortar for precooling before grinding; (3) standing turbid liquid at room temperature; (4) subpackaging liquid supernatant; (5) adding RB ( rehydration buffer) into protein conglomeration; (6) making a standard curve by 5, 10, 15, 20, 25, 30 and 35 micrograms of BSA (Bull Serum Albumin); (7) loading analysis gel, namely silver staining, with the quantity shown in the specification; (8) taking out a dried gel strip and rewarming at room temperature; (9) adding coverage oil into an isoelectric focusing disk; (10) performing isoelectric focusing treatment; (11) rewarming the treated gel strip; (12) preparing low melting agarose from upper tank liquid; and (13) carrying out coomassie blue staining on the gel and drawing. The method is easy to implement and is suitable for extracting of rat hippocampus tissue protein samples, the operation is simple and convenient, and a dimensional electrophoretogram with more protein spots, clear horizontal stripes and good repeatability is obtained. |
| priorityDate | 2014-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.