http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103882142-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2014-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103882142-B |
titleOfInvention | A kind of method of Rapid identification and evaluation plant reference gene suitability |
abstract | The invention discloses a kind of method of Rapid identification and evaluation plant reference gene suitability, comprise the steps: that (1) determines target plant kind and target gene, extract the total serum IgE of target plant, what reverse transcription was cDNA as PCR is template, select multiple reference gene, design Auele Specific Primer carries out real-time fluorescence quantitative PCR and is obtained by reacting CT value; (2) described CT Value Data is passed through geNorm, NormFinder, BestKeeper tri-kinds of its suitabilities of software analysis, with the RankAggreg routine package of R software, obtain in each process four groups of suitability sorting datas are weighted average polymerization, obtain the definitive application sequence of described multiple gene.The method of the invention for promote to disclose the basic research such as growth and development of plants, degeneration-resistant mechanism and cultivate fast-growing, the applied research tool such as new variety of plant that is degeneration-resistant, high yield is of great significance. |
priorityDate | 2014-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 247.