http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103882002-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-08 |
| filingDate | 2014-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103882002-B |
| titleOfInvention | The preparation of a kind of immobilization proteinase reagent and application thereof |
| abstract | The invention discloses preparation and the application thereof of a kind of immobilization proteinase reagent.A kind of immobilized enzyme disclosed by the invention is made up of hydrophobic carrier material and the protease being fixed on described hydrophobic carrier material.The magnetic nano particle immobilized trypsin that the two kinds of polymer chains of different nature hydrophilic, hydrophobic utilizing surface Atom Transfer Radical Polymerization method (SI ATRP) to prepare respectively disclosed by the invention are modified, achieve the enzymolysis quick, efficient, comprehensive of albumen, and immobilization proteinases different for two kinds of hydrophilic, hydrophobic character is used in combination, achieve complementary enzymolysis, can effectively reduce the enzymolysis bias that carrier selectivity causes, improve the comprehensive of protein digestion, thus dramatically increase the qualification quantity of protein and peptide fragment. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104911162-B |
| priorityDate | 2014-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 112.