http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103881959-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-46 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-36 |
| filingDate | 2014-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103881959-B |
| titleOfInvention | A kind of tilapia source streptococcus agalactiae virulence method for weakening |
| abstract | The invention discloses a kind of tilapia source streptococcus agalactiae virulence method for weakening, by being separated, the wild strain preserved is streak culture at blood agar, chooses single bacterium colony in TSB substratum 25 DEG C of shaking culture; Step 1: be inoculated in new TSB substratum 25 DEG C of shaking culture after cultivating 6h, 6h passes a generation and reached for 20 generations; Step 2: get the 20th culture thing and be inoculated in new LTSB substratum 35 DEG C of shaking culture, 6h passes a generation and reached for 40 generations; Get the 41st generation repetitive operation step 1 to 60 generations; Get the 61st generation repetitive operation step 2 to 80 generations; Continue according to the method described above to go down to posterity, in succeeding generations, the virulence of bacterial strain is measured, go down to posterity until determine that Strain Virulence weakens.The present invention has simple and quick feature, application the method to tilapia source streptococcus agalactiae wild strain virulence weaken the low virulent strain virulence obtained weak, not easily return poison, the method is the significant and using value of the exploitation of tilapia streptococcicosis attenuated vaccine. |
| priorityDate | 2014-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.