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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28
filingDate 2014-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a5a00640b8446b107ca97c6b18a98ef
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publicationDate 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103868778-A
titleOfInvention Method for measuring lignin content of herbaceous plants
abstract The invention relates to a method for measuring the lignin content of herbaceous plants. The method comprises the following steps: (1) drying, smashing and sieving a plant sample to obtain plant sample powder; (2) adding acetic acid to the plant sample powder to obtained fully-softened plant sample powder; (3) carrying out centrifugation to obtain a precipitate A; using the acetic acid to clean the precipitate A; (4) embathing the precipitate A into an ethanol-ether solution to obtain a precipitate B; (5) after the precipitate B is dried by distillation, adding sulfuric acid for stirring to be uniform, and then standing at room temperature; (6) adding distilled water for stirring to be uniform; after boiling water bath and cooling at the room temperature, sequentially adding the distilled water and barium chloride to shake up for centrifugation to obtain a precipitate C; (7) after the precipitate C is flushed with water, sequentially adding the sulfuric acid and potassium dichromate, and cooling to the room temperature after reaction; (8) transferring a substance obtained in the step (7) into a conical flask; (9) after adding potassium iodide into the conical flask for reaction, adding a starch indicator, using a sodium thiosulfate solution to titrate to a terminal point; (10) carrying out a blank test; (11) calculating the lignin content. The method is simple, convenient and accurate.
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priorityDate 2014-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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