| abstract |
The invention discloses a polymerase chain reaction (PCR) detection method of porcine eperythrozoon, and the method successively comprises the steps of pathogen separation and purification, porcine eperythrozoon DNA extraction, primer design and synthesis, ordinary PCR detection, positive plasmid standard substance preparation and real-time fluorescent quantitative PCR detection. Compared with IHA (indirect hemagglutination assay), ELISA (enzyme-linked immuno sorbent assay) and other detection methods, the method has the advantages of high specificity, high sensitivity, high accuracy and reliability, can fast, simply, reliably and accurately detect eperythrozoon in a sample, and can also be used for epidemiological investigation and curative effect detection of eperythrozoonosis. |