http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103865989-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2012-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103865989-B |
titleOfInvention | Tnf gene mutation detection specific primer and liquid-phase chip |
abstract | The invention discloses a kind of tnf gene sudden change and detect liquid-phase chip and Auele Specific Primer, this liquid-phase chip mainly includes: every kind of tag sequence and 3 ' by 5 ' end is held the ASPE primer forming for the specific primer sequence in genes of interest mutational site, does is described specific primer sequence: for the SEQ in G8226A site ID NO.15 and SEQ ID NO.16, for the SEQ in G-308A site ID NO.17 and SEQ ID NO.18, for the SEQ in A1031G site ID NO.19 and SEQ ID NO.20, for the SEQ in G863T site ID NO.21 and SEQ ID NO.22, for the SEQ in G857A site ID NO.23 and SEQ ID NO.24, for the SEQ in G8146C site ID NO.25 and SEQ ID NO.26, and/or for the SEQ in C5478T site ID NO.27 and SEQ ID NO.28, there is the coated microballoon of different anti-tag sequences, amplimer. the identical rate of the testing result of detection liquid-phase chip provided by the present invention and PCR sequencing PCR, up to 100%, realizes wild type and the saltant type parallel detection in multiple mutational sites. |
priorityDate | 2012-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.