http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103865956-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0793 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2013-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103865956-B |
| titleOfInvention | Utilize the method that recombinant slow virus induced nerve stem cells breaks up to dopaminergic neuron |
| abstract | The present invention relates to a kind of biotechnology, a kind of method utilizing gene recombined virus induced nerve stem cells to break up to dopaminergic neuron: recombined lentivirus vector builds；The separation of neural stem cell, cultivate and identify；Recombinant virus transfection NSCs；The qualification of recombinant slow virus transfection NSCs；Recombinant virus transfection NSCs: take the digestion of the NSCs after passing on 3 times and be prepared as single cell suspension, be seeded in culture plate；Transfection TH+Brn4 gene recombinaton slow virus；Add slow virus and ploybrene, after rocking mixing, culture plate is put in incubator and cultivate, after 12 hours, change fresh division culture medium；Screen with puromycin, to obtain the cell of stable transfection simultaneously；It is an advantage of the current invention that: by building the slow virus carrier of genes of interest TH and Brn4, after transfection NSCs, allogenic gene can be expressed at cell inner stablity；A high proportion of TH positive dopaminergic neuron is produced after differentiation；Brn4 can promote the high expressed of neurotrophic factor, and has the function of inhibited apoptosis. |
| priorityDate | 2013-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 235.