http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103865940-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-15 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
filingDate | 2014-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103865940-B |
titleOfInvention | A kind of ILE '-hydroxylase gene and genetic engineering bacterium and application |
abstract | The invention belongs to biological technical field, be specifically related to the ILE '-hydroxylase gene of one catalysis ILE generation specifically (2S, 3R, 4S)-4-hydroxyisoleucine, and the genetic engineering bacterium that contains this gene. Utilize this bacterial strain to produce (2S, 3R, 4S)-4-hydroxyisoleucine, can overcome that the recovery rate that extraction method exists is low, separation and purification is difficult, Raw Material Demand amount is large, high in cost of production is not enough; Chemical synthesis severe reaction conditions, the problem that step is many, separation difficulty yield is low; And enzymatic conversion method rate is low and the problem that contains the accessory substances such as a large amount of butyrines, make only to exist in zymotic fluid (2S, 3R, the 4-hydroxyisoleucine of 4S)-a kind of configuration of 4-hydroxyisoleucine, and further improve transformation efficiency by the method that ILE hydroxylase encoding gene is crossed expression, thereby reach the object of simplifying technique, reducing production costs. |
priorityDate | 2014-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 340.