http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103865926-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
filingDate | 2014-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103865926-B |
titleOfInvention | IL-17 fluorescent quantitative PCR detection method in a kind of pig intestinal tissue |
abstract | The present invention relates to IL-17 fluorescent quantitative PCR detection methods in a kind of pig intestinal tissue.This method includes design specificity fluorescent quantification PCR primer; establish quantitative fluorescent PCR reaction system and response procedures; the plasmid that preparation contains target fragment IL-17 draws standard curve as standard plasmid; the target gene and reference gene GAPDH of last sample to be tested expand simultaneously, according to relative quantification △ △ C T The expression quantity of method detection gene.The present invention is due to being utilized quantitative fluorescent PCR with high sensitivity, high specificity, the characteristics of pollution-free and real-time, accurate and fast, for detect chitling road position IL-17 expression, so that auxiliary evaluation animal intestinal tract position immune state or lysis provide a practical approach. |
priorityDate | 2014-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 193.