http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103864914-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2014-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103864914-B |
| titleOfInvention | The preparation method of high-purity interleukin II 4 inclusion body |
| abstract | The present invention relates to the preparation method of a kind of high-purity interleukin 24 inclusion body, comprise the following steps: the preparation recombination bacillus coli containing interleukin 24 gene；Recombination bacillus coli carries out fermentation culture；The thalline obtained after fermentation culture carries out ultrasonication and obtains inclusion body；Inclusion body is washed；Inclusion body after washing is carried out degeneration and renaturation, obtains interleukin 24 albumen.Compared with prior art, the present invention is optimized by escherichia coli are produced interleukin 24 condition of culture, obtain more loose interleukin 24 inclusion body structure, improve the efficiency of albumen renaturation in higher concentrations, simultaneously, the inventive method makes the degeneration efficiency of interleukin 24 and annealing efficiency be respectively increased 50.52% and 83.78%, simple to operate, has saved production cost.This simplify the method for its purge process by optimum culture condition and be applicable to other albumen having similar inclusion body construction features. |
| priorityDate | 2014-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 112.