http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103849657-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-64 |
filingDate | 2014-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103849657-B |
titleOfInvention | A kind of production method of biofuel grease |
abstract | A kind of production method of biofuel grease, as follows: microorganism is carried the rear surplus materials cytase enzymolysis of oil by (1), obtain microorganism carry oil after surplus materials enzymolysis solution (2) by water and the above-mentioned enzymolysis solution mass ratio by 5 ~ 3:1, and add magnesium sulfate, iron(ic) chloride and SODIUM PHOSPHATE, MONOBASIC, preparation heterotrophic microorganism fermention medium; (3) produce oil heterotrophic microorganism is inoculated in three stage fermentations in the fermention medium of step (2); Produce oil autotrophic microorganism is inoculated in three stages cultivation in sterilizing f/2 substratum through aseptic technique; (4) oleaginous microorganism biomass in step (3) are collected, first pre-treatment, press normal hexane, ethanol and pretreated microbial biomass volume ratio 1:1:1 ~ 3 again, extract grease, organic phase through 30 ~ 50 DEG C, 20 ~ 80 revs/min of rotary evaporations, desolventizing, impurity elimination, dry, obtain microbial oil.Invention increases produce oil efficiency, reduce production cost, environmental friendliness, save the area, protection of the environment. |
priorityDate | 2014-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.