http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103849593-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2012-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103849593-B |
| titleOfInvention | A kind of Magneto separate formula cell three-dimensional co-culture method |
| abstract | The present invention relates to regenerative medicine field, be a kind of Magneto separate formula cell three-dimensional co-culture method.Inducing cell and magnetizing mediums microgranule are embedded in sodium alginate micro gel capsule by the present invention jointly, this microcapsule carries out non-contact 3-D in same system with the calcium alginate micro gel bead being embedded with stem cell again and co-cultures, utilize the soluble factor of induced cellular secretion in microcapsule that the directed differentiation of stem cell is regulated and controled, at the end of co-culturing, use magnetic field that microcapsule is separated with micro gel bead, and utilize sodium citrate solution solubilized micro gel bead, gather in the crops pure noble cells further.The present invention is simple to operate, low cost, microcapsule and micro gel bead can be the three dimensional growth environment in cell provides approximation, microcapsule membrane can make the inducible factor of induced cellular secretion act on stem cell through microcapsule, and realizing the immunity isolation of dissimilar cell simultaneously, magnetizing mediums microgranule and magnetic separating device are then conducive to the separation of cell and results after inducing cell and differentiation. |
| priorityDate | 2012-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.