http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103837593-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-626 |
filingDate | 2014-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103837593-B |
titleOfInvention | Isotope dilution mass spectrometry quantitative approach after a kind of human serum protein electrophoresis |
abstract | The invention discloses isotope dilution mass spectrometry quantitative approach after a kind of human serum protein electrophoresis, belong to Protein quantitative analysis technical field, including: native polyacrylamide gel electrophoresis (PAGE) separates transferrins, albumin in human serum, and " isotopic dilution after electrophoresis " method of exploitation adds enriched isotope on protein band 34 S diluent, in LA ICP MS detection protein band 32 S + / 34 S + Ratio, carries out quantitative analysis to transferrins, albumin in human serum.After the electrophoresis that the present invention sets up, isotopic dilution quantitative approach preparation of reagents is simple, process is simple and efficient, without preparing specificity diluent, and the application that quantitative approach is in PAGE LA ICP MS multiple techniques overcome that specificity diluent limitednumber in current quantification of protein technology brings a difficult problem for some quantification of protein can only be promoted the use of the quantitative analysis of most protein in complex sample. |
priorityDate | 2014-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 875.