http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103834715-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9bee93582e9da8a97df9bdd392d70582 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2014-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9fe22c865b3e2a15b82efde0cc2f7ac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb4f54b9edd9199ec65bfb517c9f5e22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8ab03a107fc84839ff407c252dc9d0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bfe948b2db562c9711186756452cf97 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58e864a9767fdc1b7aba709c5425b60c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e819d83e010005ea506cc954a9fcf3f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40110eb04801d309237a2d8815dbf67c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39e72e6176e835761ef5c7edb3caefe8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0b22c09a8576979853ad4cf06b21563 |
| publicationDate | 2015-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103834715-B |
| titleOfInvention | WST-8 (water soluble tetrazolium-8) testing method of in vitro cytotoxicity of tobacco juice of electronic cigarette |
| abstract | The invention relates to a WST-8 (water soluble tetrazolium-8) testing method of in vitro cytotoxicity of a tobacco juice of an electronic cigarette. The method comprises the following steps: 1) preparation of an experimental reagent; 2) cell inoculation; 3) contaminating of the tobacco juice of the electronic cigarette; 4) dyeing by WST-8; and 5) result and analysis. Compared with the prior art, the method provided by the invention has the characteristics that the testing step of washing the cells for many times in the whole testing process is not required, so that the operation is simpler and more convenient; the step of changing a culture solution is cancelled during WST-8 dyeing, so that a diluted culture solution can be directly added to react; formazan generated by the WST-8 is water soluble, so that the subsequent dissolving step is canceled, and the light absorption value detection can be realized within 1.5-2 hours after dyeing; the testing period is short and the testing method is convenient and fast. As the WST-8 is non-toxic to the cells, the testing method has the advantages of no damage to the cells, high detection flux, high sensitivity, accurate quantification and the like. |
| priorityDate | 2014-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.