http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103834641-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2013-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103834641-B |
| titleOfInvention | A kind of molecular specificity of resistance straw mushroom bacterial 0229 detection mark and its detection method |
| abstract | The present invention relates to a kind of molecular specificity of resistance straw mushroom bacterial 0229 detection mark and its detection method, it is characterised in that：The specific detection is labeled as specific primer, and wherein primer sequence is：F：CTTTCAGGAAGAGCCCAGC；R：TGAAGGGCTTGAAGGAGAAA.Detection method, including：Resistance straw mushroom bacterial is inoculated with PDA plate culture medium, 32 DEG C of culture 5d, scraping surface mycelia is fitted into sterile centrifugation tube, and 70 DEG C of refrigerators are preserved, and are then extracted genomic DNA with improved CTAB methods, are obtained DNA；It is template by above-mentioned DNA, specific detection labeled primer is amplimer, enters performing PCR amplification, obtain pcr amplification product, carry out electrophoresis detection.Detection time of the present invention is short, the high advantage of accuracy, utilizes obtained molecular labeling to differentiate resistance straw mushroom bacterial 0229, is conducive to the development of straw mushroom breeding work, accelerates the R＆D work of straw mushroom new varieties. |
| priorityDate | 2013-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.