| abstract |
The invention discloses low-temperature xylanase XynAGN16 and a gene thereof, a recombinant vector and a recombinant strain. The invention provides xylanase XynAGN16 from arthrobacter sp., wherein the amino acid sequence of xylanase XynAGN16 is shown by SEQ ID No.1; the invention also provides a coding gene xynAGN16 of the xylanase and a recombinant vector and a recombinant strain of the xylanase gene xynAGN16. The xylanase disclosed by the invention has the following properties: the optimal pH is 6.5, the optimal temperature is 45 DEG C, and the enzyme activity is 17.2%, 27.6% and 41.8% at 0 DEG C, 10 DEG C and 20 DEG C respectively; after one-hour treatment at 60 DEG C, the remaining enzyme activity is 23.3%, and the xylanase is more stable than other low-temperature xylanase. The xylanase disclosed by the invention can be applied to the feed and food industries. |