http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103822906-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2014-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103822906-B |
| titleOfInvention | A kind ofly quantitatively detect the method for spike for HAP nano particle in cell |
| abstract | The present invention relates to a kind of hydroxyapatite nano particle quantitative detecting method based on rare-earth fluorescent signal, it is characterized in that including following steps: the fluorescence-concentration standard curve 1) formulating europium ion; 2) set up the stripping ratio of calcium ion and europium ion in HAP nano particle and determine the accuracy of fluorescence-concentration standard curve method; 3) in cell, HAP nano particle quantitatively detects; 4) HAP nanoparticle dissolution fate elucidation in cell.Major advantage of the present invention is: 1) good biocompatibility, safe and reliable, does not exist the problem that radioactive label service condition is limited; 2) detection limit is low, and detection limit concentration can reach 0.5nM, and required sample size is few; 3) react HAP nano particle more accurately in intracellular dissolving situation, the stripping of europium ion and the dissolving matching of HAP nano particle high. |
| priorityDate | 2014-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.