http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103820578-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5610690048edbc271e03b26b23f76ff9 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-113 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2010-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a5a07e279e65a5b9c6d0ad02f79f2b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e77883bd9d6a9c0e132fab4dae36565 |
| publicationDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103820578-B |
| titleOfInvention | Method and kit for detection of microorganism |
| abstract | The application relates to a method and a kit for detection of microorganism. The living cells of microorganisms in a test sample are distinguished from dead cells or damaged cells and detected by means of the following steps: a step (a) in which the test sample has added thereto a pharmaceutical preparation that covalently bonds with DNA or RNA upon being irradiated with light having a wavelength of 50-700nm; a step (b) in which the test sample with a crosslinking agent added thereto is irradiated with light having a wavelength of 350-700nm; a step (c) in which a target area of the DNA or RNA of the microorganisms in the test sample is amplified without extracting the nucleic acid from the cells, in the presence of a pharmaceutical preparation that suppresses the action of a nucleic acid amplification inhibitor, and by using a nucleic acid amplification method; and a step (d) in which the amplification product obtained therefrom is analyzed. |
| priorityDate | 2009-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 934.