http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103820494-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2014-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103820494-B |
| titleOfInvention | A kind of remove random integration site-specific integration carrier and construction process and application |
| abstract | The invention discloses a kind of remove random integration site-specific integration carrier and construction process and application, do you comprise pUC? ori, attB sequence and multiple clone site MCS, a loxP sequence and a rox sequence is respectively equipped with in attB sequence upstream and downstream, CMV promoter is connected to the upstream of a loxP sequence, negative screening-gene is connected with a rox sequence, negative screening-gene downstream is also connected with internal ribosome binding site IRES2, IRES2 downstream is fluorescent marker gene, fluorescent marker gene downstream is MCS, MCS upstream and downstream is respectively equipped with the 2nd rox sequence and the 2nd loxP sequence, and it is identical with a loxP sequence direction with a rox sequence respectively, MCS downstream is for just to screen element.The new application of the present invention for providing rejecting random integration reconstitution cell directly to filter out the reconstitution cell of pseudo attP site specific integration in cell screening. |
| priorityDate | 2014-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 158.